Team:Macquarie Australia/Protocols/PCR

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Polymerase Chain Reaction


PCR mixture

4.0ul 5x phusion buffer
0.4ul dNTPs
0.6ul DMSO
0.2ul Polymerase
11.8ul water
Total 17ul
1ul forward primer
1ul reverse primer
1ul template


PCR settings

Initial denaturation 98°C for 30 secs
Followed by 30 repeats of:
Denaturation 98 °C 10 secs
Annealing 60°C 10 secs
Extension 72°C 2 mins
Final extension 72°C 10 mins

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