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Team:Calgary/Sandbox/Notebook/Protocols
From 2013.igem.org
Protocols
General Protocols
- Agarose Gel Electrophoresis
- Bacterial Transformation
- Bradford Assay
- Freeze-Thaw Protocol
- Gel Extraction
- Gel Shift Assay
- Glass Beads Cell Lysis Protocol for Protein Samples
- LB Agar Plates
- Overnight Cultures
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, and Ligation
- SDS PAGE Gel
- Verification Digest
