Our goals are to stabilize the mRNA and as a result, improve the expression of a gene, or rather mamK. We used GFP-LVA as a reporter to examine the expression of a gene. By measuring the fluorescence, we can get the information about how our device worked. Look at this table:





From this data, we can see, there is obvious improving of the fluorescence, indicating the improving of the expression. Because we use the same RBS and promoter, we think the transcriptional and the translational efficiency are the same. So we think, this is a succinct evidence of the increasing mRNA level.

But, it is not direct and we only perform two experiments because it is so different to lend a microplate reader. Next, when we come back to school, we will perform RT-PCR for further experiment and provide another evidence.

We also have another two experiments groups, experiment 1(both with K1059003 and k1059004), and experiment groups 2 (with part k1059005). But, there’ something wrong with it’s sequence in experiment 1. And in experiment 4, there is a obvious decreasing of the RFU, we think may be the extra ribosome effect the binding efficiency of the real ribosome.