Prussian Blue Ferritin Synthesis

Reagents and Materials

• Horse Spleen Ferritin (47mg/mL)
• Hydrogen Chloride (pH 3.0)
• K₄Fe(CN)₆ (0.025M)
• Sodium acetate-acetic acid buffer (pH 3.6)

1. Add 20µL horsespleen ferritin (47mg/mL) to a solution of 20mL HCl(pH 3.0) and 150µL K₄Fe(CN)₆ (0.025M).
2. Dialyze for 12 hours at 4℃ while constantly stirring
3. Dialyze 5mL of the previous ferritin solution against 500mL of sodium acetate-acetic acid buffer (pH 3.6) for 8 hours.
4. Replace buffer and dialyze overnight