Team:Paris Saclay/Protocols

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Labwork Overview Biobricks Protocols Safety

Protocols

Preparation of super competent E.coli cells

Transformation of super competent E.coli cells

Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue

Plasmidic DNA Mini and Maxi preparation

PCR for the bacterial genomic DNA

Electro-elution

Gibson Assembly method

Gel Electrophoresis

Ethanol Precipitation

Digestion and Ligation

[http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]

Protocols written by Sheng (the 4 first), Nadia (Transduction) and Abdou

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