Team:Paris Saclay/Protocols

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Labwork Overview Biobricks Protocols Safety

Protocols

Preparation of supercompetent E.coli cells

Transformation of supercompetent E.coli cells

Extraction of the bacterial genomic DNA by using NucleoSpin® Tissue

Plasmidic DNA Mini and Maxi Preparation

PCR for the bacterial genomic DNA

Gibson Assembly Method

Gel Electrophoresis

Ethanol Precipitation

Digestion and Ligation

[http://www.mn-net.com/tabid/1452/default.aspx Gel Purification ]

Protocols written by Sheng (the 4 first), Nadia (Transduction) and Abdou

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