Team:BYU Provo/Notebook/Cholera - Enzyme/October/Period2/Dailylog

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Cholera - Enzymes Notebook: October 15 - October 31 Daily Log



Cholera Enzyme
March-April
May-June
July-August
September-October
Protocols

10/15/13

We ran a gel for the colony PCR products we started last night and colony 5 showed a bright band right around 1100 bp's which is the right size for DspB. We then started a 5 ml overnight in LB-Amp from its corresponding streak on the plate.


10/16/13

We ran plasmid purification on our DspB Colony E from the O/N we started yesterday. We then transformed the plasmid into BL21, which is the E. coli strain that we use for expression and purification. The transformation product was plated on LB+AMP and left in the 37° incubator overnight. All procedures were performed according to the protocols listed on our protocols page.


We also made 500 mL of LB in preparation for growing up our BL21 with DspB. The plasmid specific antibiotic, AMP, will be added tomorrow after the LB is autoclaved and ready for use.



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