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PCR and gel electrophoresis analysis

PCR

Principle

Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:

• First denaturation
  
• Denaturation step
  
• Anealing step
  
• Elongation step
  
• Final step
  

The 2nd, 3th and 4th steps are repeated 20-40 cycles.

Preparation

Optimisation

Number of primer

Temperature

Gel electrophoresis analysis

Principle

Preparation

Analysis

Site developped by Gabriel Guillocheau