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Team:CU-Boulder/Notebook/Protocols
From 2013.igem.org
- Embedding PDF Files
- In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
- Embedding Google Docs
- Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
- Chemically Competent Cells
- This protocol details how to prepare chemically competent cells.
- Experiments
- Experiment 2.1 - Competent Cell Efficiency
- Chemical Transformations
- In this protocol we cover how to use our chemically competent cells to perform transformations.
- Plasmid Minipreps
- Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
- Restriction (Double) Digests
- Our method of performing restriction double digests is detailed in this protocol.
- Homemade Buffer Compositions
- The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.
- Recycling DNA Mini-columns
- This protocol covers how to recycle mini-columns used in plasmid miniprep systems.
