Team:Evry/Protocols/11
From 2013.igem.org
PCR Purification
Goal
Preparation
Protocol adapted from Thermo Scientific PCR Purification notebookGoal
The aim of the purification step is to recover the plasmid produced by the bacteria (cloning strain).
The different step are use to throw other bacterial components off (proteins, cell wall, genomic DNA, etc).
Preparation
Protocol adapted from Macherey-Nagel plasmid purification notebook1.
2.
3.
4.
5.
6.
7.
8.
Test