Team:Bielefeld-Germany/Biosafety/Biosafety System L

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Safety system L (leaky)


IGEM Bielefeld 2013 biosafety lacI.png
bablablablablablablablablabla.....

IGEM Bielefeld 2013 biosafety Rhamnose-promoter.png



IGEM Bielefeld 2013 biosafety alr.png



IGEM Bielefeld 2013 biosafety Terminator.png



IGEM Bielefeld 2013 biosafety dlac-promoter.png



IGEM Bielefeld 2013 biosafety RNase Ba.png




System L in the MFC: In this case the mikroorganism is in the MFC with sufficient L-rhamnose. It comes to an expression of lacI which blocks the lac-promoter by binding and alr which switches L-alanine to D-alanine. Because of the fact that lacI blocks the lac-promoter the RNase Ba can't expressed.




System L outside of the MFC: In this case the mikroorganism could get out of the MFC by damage or incorrect handling. Outside of the MFC there isn't enough L-rhamnose. So... E.coli dies.
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