Notebook : July 5

Summary:

For fnr promoter regulator system:

• Stocked 2 samples for each 2 colonies which contained AmilCP or BBa_K1155000.
• Performed restriction digest for amilCP and fnr from the medium seeded yesterday.
• Verification DNA quantity by NanoDrop.
• Performed electrophoresis and photo interpretation .

For PCBs sensor system:

• Stocked 2 samples of pseudomonas KE707. and performed a PCR
• Received Primer BphA1P_up/down and BphR2_up/down.

Lab work

• A.aero/anaerobic regulation system
  • 2.BioBrick RBS+LacZ+terminator in plasmid PSB1C3
  • BioBrick RBS+amilCP+terminator in plasmid PSB1C3

Electrophoresis band size estimation

We used Clonemanager for band size estimation:

DNA quantification
Result(average):

• fnr promoter : 761ng/µl
• AmilCP : 229ng/µl

By using NanoDrop, we found some erreurs which could be due to device malfunctional issue.

Electrophoresis

• PCBs sensor system
  • 1.BioBrick BphR2(regulator) in plasmid PSB1C3