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Team:TU-Munich/Notebook/Methods
From 2013.igem.org
Molecular-biological Methods
Preparation of Nucleotide fragments
Digestion, Dephosphorylation
Hybridization of Oligonucleotides
Polymerase Chain Reaction
Site-directed Mutagenesis
Agarose Gel Electrophoresis
Ligation of DNA-Fragments
Microbiological Methods
Preparation of LB-Media
LB-Media | |
| Reagent | Amount |
Transformation of E. coli
Cultivation of E. coli
Protein-biochemical Methods
Physcomitrella Methods
The techniques used for the cultivation and manipulation of Physcomitrella patens are based on the knowledge of the chair for Plantbiotechnology of Prof. Dr. Reski at Freiburg University which are availible at [http://www.plant-biotech.net Plant-Biotech.net]
Preparation of Knob-Media
Knob-Media | |
| Reagent | Amount |
Cultivation of P. patens
Homogenization
Bioinformatical Methods
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