Team:ZJU-China/Notebook/LabNotes/June

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Lab Notes: June

Date Notes
June 1stGel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P.
June 2ndGel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP).
June 3rdPick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time.
June 4thPick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st.
June 5thFind monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010.
June 6thSmall-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes.
June 7thPlasmid purification of 23L part. Store strains in -80°C.
June 8thTransformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP.
June 9thSmall-scale plasmid purification of GFP.
June 10th
June 11thPreliminary experiment using new competent cell of DH52, BL21, and ???????
June 12thPick up colonies of GFP and R0010 and cultivate in LB+A medium.
June 13th-30thFinal examinations for the semester.