Team:ZJU-China/Notebook/LabNotes/June

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Lab Notes: June

Date Notes
June 1Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P.
June 2Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP).
June 3Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time.
June 4Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st.
June 5Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010.
June 6Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes.
June 7Plasmid purification of 23L part. Store strains in -80°C.
June 8Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP.
June 9Small-scale plasmid purification of GFP.
June 10
June 11Preliminary experiment using new competent cell of DH52, BL21, and ???????
June 12Pick up colonies of GFP and R0010 and cultivate in LB+A medium.
June 13-30Final examinations for the semester.