Verification Digest

Reagents and Materials

• Restriction enzymes
• Appropriate buffer
• Plasmid purified DNA
• 1% agarose gel

Protocol

1. In a tube, add (per reaction):
• 4µL Plasmid purified DNA
• 1µL appropriate buffer
• 0.2µL enzyme 1
• 0.2µL enzyme 2
• 3.6µL ddH₂O (deionized water)
2. Incubate at 37 degrees Celsius for 1h
3. Run 10µL in a 1% agarose gel at 100V