Glass Beads Cell Lysis Protocol for Protein Samples

Reagents and Materials

• 0.1m diameter glass beads
• Lysis buffer
• Overnight induced cultures
• 1.5mL tubes

Protocol

1. Culture and induce protein-producing bacteria
2. Centrifuge cells at max for 10 minutes. Discard supernatant
3. Resuspend cells in 2mL of lysis buffer
4. In a 1.5mL tube, add ~0.5mL of glass beads and 1mL of cells resuspended in lysis buffer
5. Leave on ice for 5 min. Leave on BeadBeater or Vortex for 5 min. Repeat this cycle 6 times
6. Centrifuge at max for 20 minutes
7. Transfer supernatant (crude lysate) to a new tube and discard the glass beads