Team:BYU Provo/Notebook/Cholera - Enzymes/September-October

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Cholera - Enzyme Notebook September-October



Cholera Enzyme
March-April
May-June
July-August
September-October
Protocols

September 1 - September 14


We successfully prepared the DspB and AmyA plasmids to submit to the iGem registry and those to be used in our protein purification. We then were able to purify DspB and AmyA to use in our biofilm assay.


Daily log


Protein GelAMYA927.jpg
September 15 - September 30


We focused on running our biofilm assay and getting data on the degradative enzymatic activity of the DspB and AmyA proteins we have purified.


Daily log


BYU2013-CholeraGroup2.jpg
October 1 - October 14


We went to the North American iGem Jamboree and did well enough to make it to the World Championships in November. We got back to the lab extremely excited and motivated. We worked on finalizing our DspB plasmid to get it ready to over express and purify in E. coli.


Daily log


Protein GelAMYA927.jpg
October 15 - October 31


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Daily log

Protein GelAMYA927.jpg