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PCR Purification

Goal

The aim of the PCRpurification step is to recover DNA from PCR and other enzymatic reacti mixtures.

Preparation

Protocol adapted from Thermo Scientific PCR Purification notebook

1.
Add 1:1 volume of Binding Buffer to completed PCR mixture (e.g. if you have 100 µL of reaction mixture, add 100 µL of Binding Buffer). Mix thoroughly and check the solution's color: a yellow color color indicates an optimal pH for DNA binding.

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Test