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User contributions
From 2013.igem.org
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- 15:10, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 15:09, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 15:07, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 15:06, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 15:04, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Team
- 14:44, 17 August 2013 (diff | hist) Team:NU Kazakhstan
- 13:27, 17 August 2013 (diff | hist) N 17 August 2013 (Created page with "Today we did ultrapurification of dsDNA from SELEX 11 that was PCR amplified yesterday (see protocols for details). We used 100 ul of DNA in order to get 50 ul of purified DNA. H...") (top)
- 15:03, 8 August 2013 (diff | hist) Team:NU Kazakhstan
- 15:08, 22 July 2013 (diff | hist) N 22 July 2013 (Created page with " We have observed the cultures growing after plating the transformed bacteria. We took some colonies and put them for incubation in LB broth, and left for incubation overnight at...") (top)
- 16:53, 19 July 2013 (diff | hist) Team:NU Kazakhstan/Team
- 16:50, 19 July 2013 (diff | hist) Team:NU Kazakhstan/Team
- 16:48, 19 July 2013 (diff | hist) Team:NU Kazakhstan
- 12:35, 18 July 2013 (diff | hist) N 18 July 2013 (Created page with "<html> <div>TOPO TA cloning was performed for two PCR products, aptamers from SELEX cycle 11: one is with A-overhangs and another is without overhangs.</div> <ul><b>Ligation reac...") (top)
- 12:15, 18 July 2013 (diff | hist) N 17 July 2013 (Created page with "<html> <div>Today we decided to amplify DNA from SELEX 11 with PCR. In addition, this will be DNAs will contain A-overhangs in order to be suitable for TOPO TA cloning.<div> <ul>...") (top)
- 15:25, 16 July 2013 (diff | hist) N 16 July 2013 (Created page with "<html> <ol> <li>No colonies grew on ampicillin plates because PCR products were not of appropriate form, and wrong protocol was used for TOPO TA cloning.</li> <li>New LB agar/Amp...") (top)
- 19:31, 14 July 2013 (diff | hist) Team:NU Kazakhstan/Attributions
- 19:29, 14 July 2013 (diff | hist) Team:NU Kazakhstan/Attributions
- 17:51, 14 July 2013 (diff | hist) Team:NU Kazakhstan
- 17:50, 14 July 2013 (diff | hist) Team:NU Kazakhstan
- 17:49, 14 July 2013 (diff | hist) Team:NU Kazakhstan
- 17:46, 14 July 2013 (diff | hist) Team:NU Kazakhstan/Attributions
- 17:43, 14 July 2013 (diff | hist) Team:NU Kazakhstan
- 17:42, 14 July 2013 (diff | hist) Team:NU Kazakhstan/Attributions
- 17:41, 14 July 2013 (diff | hist) Team:NU Kazakhstan
- 17:40, 14 July 2013 (diff | hist) Team:NU Kazakhstan/Project (top)
- 17:25, 14 July 2013 (diff | hist) Team:NU Kazakhstan
- 17:24, 14 July 2013 (diff | hist) Team:NU Kazakhstan
- 15:47, 11 July 2013 (diff | hist) N 11 July 2013 (Created page with "<html> <ol> Today we performed TOPO TA cloning of our PCR products of DNA aptamers from SELEX cycle 8. For this procedure we used pGEMTII Vector. The following steps were done: <...") (top)
- 18:41, 10 July 2013 (diff | hist) N 10 July 2013 (Created page with "<html> <div>Today, we checked the products of SELEX cycle 8 in order to proceed to cycle 9. We amplified the products using different number of cycles in PCR machine: 10, 12, and...") (top)
- 19:00, 8 July 2013 (diff | hist) Team:NU Kazakhstan/Team
- 18:49, 8 July 2013 (diff | hist) Team:NU Kazakhstan/Team
- 18:46, 8 July 2013 (diff | hist) Team:NU Kazakhstan/Team
- 18:40, 8 July 2013 (diff | hist) Team:NU Kazakhstan
- 18:29, 8 July 2013 (diff | hist) Team:NU Kazakhstan
- 18:28, 8 July 2013 (diff | hist) Team:NU Kazakhstan
- 10:42, 3 July 2013 (diff | hist) N 3 July 2013 (Created page with "<html> We have analyzed dsDNA sequences from SELEX cycles 5, 6, 7, 8 by running them through gel. However, no bands appeared, probably, because the concentration of the samples w...") (top)
- 15:53, 21 June 2013 (diff | hist) N 21 June 2013 (Created page with "<html> <div>We prepared LB agar/Ampicilin and LB agar/Kanamycin plates for later cloning procedure. Cloning is done on the last round of SELEX to select individual aptamers, whic...") (top)
- 14:36, 19 June 2013 (diff | hist) N 19 June 2013 (Created page with "<html> We continued doing SELEX 8 for CEA. We have done the following: <ol> <li>precipitation</li> <li>PCR</li> <li>Checked our PCR products by running them in the gel</li> </o...") (top)
- 14:21, 19 June 2013 (diff | hist) Team:NU Kazakhstan/Team
- 11:06, 19 June 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 11:04, 19 June 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 14:26, 17 June 2013 (diff | hist) N 17 June 2013 (Created page with "<html> <div>Today, we assisted Madina (one of our advisors) to do the SELEX cycle 8 (selecting aptamers against CEA) and to finish cycle 3 of SELEX (selecting aptamers against EC...") (top)
- 15:55, 14 June 2013 (diff | hist) N 14 June 2013 (Created page with "<html> We were planning to perform Western Blot analysis in order to check the specificity of binding between CEA protein and DNA aptamers from SELEX 7th and 8th cycles. However,...") (top)
- 13:38, 13 June 2013 (diff | hist) N 13 June 2013 (Created page with "<html> <div>We did PCR of the DNA aptamers from SELEX 8, and then two samples were run in the gel in order to check if their length is 80bp long.</div> <div>We also had a meeting...") (top)
- 12:47, 11 June 2013 (diff | hist) N 11 June 2013 (Created page with "<html> <div>1. Amplified the samples extracted from the yesterday's gel with PCR</div> <div>2. Runned the amplified dsDNAs on the gel (200V, 100mA)</div> <div>3. Observed the gel...") (top)
- 14:47, 10 June 2013 (diff | hist) N 10 June 2013 (Created page with "<html> <div>1. We decided to repeat Electric Mobility Shift Assay with different concentrations of CEA protein and DNA aptamers. We decided to take Protein:DNA ratio as 1:5, i.e....") (top)
- 14:16, 10 June 2013 (diff | hist) Team:NU Kazakhstan/Team
- 15:52, 7 June 2013 (diff | hist) Team:NU Kazakhstan
- 15:26, 7 June 2013 (diff | hist) Team:NU Kazakhstan
- 15:19, 7 June 2013 (diff | hist) N 7 June 2013 (Created page with "<html> <div>We had a meeting with our advisors, and today all members of the team were present (Alexander joined us today). Mukhtar made a presentation about what we did during t...") (top)
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