Team:Macquarie Australia/Notebook
From 2013.igem.org
Notebook
Intro to the week, Work being done, "Using PCR we amplified the genes X and X"
Results (This worked, this didnt), "Gene X didnt amplify, Gene X did"
Small discussion + Whats next "We plan on then doing X to amplified gene"
With the break between semesters over, the Macquarie iGEM team returned to classes; for many of us this was the first day of iGEM 2013. We had our first meeting and discussed our project. Dr. Louise Brown and Associate Professor Rob Willows were introduced to the team and we began to determine who would take on certain roles within the team.
We eagerly began our project, deciding to use Gibson Assembly to produce our optimised genes. We had decided to develop BioBricks necessary for the biosynthesis of chlorophyll within E.coli. To do this, we constructed a gene pathway, containing 12 genes to reach our goal of chlorophyll biosynthesis.
Week 2 (G block assemblies & transformations) - Update Coming soon