Materials we have received from the Mascher research group
Escherichia coli MG1655 red (Km)
Escherichia coli MG1655 recA⁻(Tc) (PCR template: tetracycline resistance gene)
Bacteriophage P1 (for P1 transduction)
pFTGT (PCR template: FLP recombinase)
m(C2-Ap)Sm (PCR template : streptomycin resistance gene)
pBAD/Myc-HisA (PCR templat : pBAD promoter)
pSG76C (PCR template: FRT and chloramphenicol resistance gene)
pSG76A2 ((PCR template: FRT and ampicillin resistance gene)
pACYC177 (PCR template: kanamycin resistance gene)
PCR primers (sequences are not written for IPR Protection)
Cm-NR-stu
Cm-CR-stu
CmC
araC2
pBAD-C3v
TcN
TcC3
141-70
R6Kori-Xho-3R
pACYC177
araCHC
araC
CmNR-Not
CmC2
From Junichi Kato (Professor, Molecular Genetics Laboratory, Department of Biological Sciences, Tokyo Metropolitan University)
pKK009 (pilV gene+shfflon+rci recombinase, vector: pBR322)
pKK024 (rci recom binase, vector: pUC9)
pKK040 (shufflon A segment, vector: pUC9)
pKK041 (shufflon B segment, vector: pUC9)
pKK042 (shufflon C segment, vector: pUC9)
239pUC ( modified shufflon, symmetry recombination site, vector: pUC19)
pINV202 (modified shufflon, symmetry inverted recombination site, vector: pUC118 )
pDEL302 (modified shufflon, symmetry direct deletion, vector: pUC118)
pAG105 (shufflon, vector: pUC18)
From Atsuko Gyohda (visiting researcher, Plant Hormone Mechanism Laboratory, Department of Biological Sciences, Tokyo Metropolitan University) & Nobuhisa Furuya
(Assistant professor, Molecular Genetics Laboratory, Department of Biological Sciences, Tokyo Metropolitan University)
pBluescriptⅡ SK(+)
UAST-Cre
From Taro Kaneuchi (Research Assistant, Cellular Genetics Laboratory, Department of Biological Sciences, Tokyo Metropolitan University)