Rehydration of Registry DNA

Protocol

• Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.
• Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want;
• Add 10 μL of ddH₂O (deionized water);
• Let the water incubate at room temperature for 10 minutes;
• Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.