Team:TU-Munich/Team/Activities

first transformation: Volker, Ingmar, Jeff, Leonie, (Anja, Omar, ten hours trafo, meeting Freiburg team, pizza mobil, sightseeing tour at night) plating: Johanna, Andi second transformation: Ingmar, Flo, Jeff, Leonie, (Timo, twelve hours of trafo, fighting w/ busdriver)

At the end of May we joined the "Drugs in the environment" conference that was organized by the [http://www.rcds.de/ RCDS] (which is the youth organisation of the CDU, the currently governing political party in Germany) and the [http://www.vfa.de/ German Association of Research-Based Pharmaceutical Companies].
During this discussion meeting we had the possibility to meet and discuss with several experts in the field such as xxx and xx as well as with representatives of leading German pharmacy companies such as Bayer and Novartis.

The main goal of the participation on the 4th Water Research Horizon Conference “Water Scarcity and Global change” in Berlin on the 25th of June 2013 http://www.ufz.de/water-research-horizon Water Research Horizon Conference was to inform ourselves about worldwide water scarcity and global change to get inspirations in for our outreach and human practice effort. Moreover we were interested in the worldwide practical realization of minimization of “the water problem” in different countries in diverse ways of use and transformation (cf. Figure 3) to see how our project fits into this scope. In addition to the main speeches of ambassadors from different communities and companies we participated on a workshop session with speeches and discussion called “Water and Soils for Food and Energy - A Question of Technological Development?”.

We realised that it is quite difficult to give a straight answer to this question. J. Bouma (formerly Wageningen University, The Netherlands) answered it with “No, not alone, but start with existing technology and go from there” whereas R. Ardakanian (United Nations University, Dresden) just said “yes” after his speech. But not only the answers to the topic question were different also the point of view concerning to the worldwide water problem, scarcity or the definition of water differed by the speakers. Water in connection with waste was the main focus of the speech of R. Ardakanian, who said that waste is a resource for production of food and energy and must contribute as recycled waste in technology. According to E. Adar the goal for the next generation was (Ben Gurion University, Negev, Israel) the challenge to minimization of water costs and use. To him it was evident that water is a synonym for food and a commodity, not a resource like gold. Furthermore M. Lange (The Cyprus Institute, Nicosia) presented his speech titled "Ways about Climate Change and Energy-Water Nexus in the Mediterranean". Eventually A. Porporato (Duke University, NC, USA) gave a lecture on the role of ecohydrologic variability on the productivity and sustainability of ecosystems. General speeches about the water scarcity and climate change also informed the audience about different present projects to reduce the water scarcity. G. Teutsch (UFZ Leipzig) who opened the conference talked about a “quick start” concerning to reduce the water scarcity which sticks together with the rise of climate change. The main statement of the speech from the second speaker P. Krebs (TU Dresden) was that not visions, only excellent concepts, developed new topic projects and community action yield viable results. That the water topic has also an important role in politic was approached from F. Holzwarth (BMU Bonn) who also had the goal of forming a “water alliance with new individual faces”. In his view in contrast to the climate change the water scarcity isn’t a major topic in politic based on the fact “disasters drive politic”. The main points of the speech from U. Weber (DFG Bonn) in case of the water scarcity and climate change were science (project, implementation and ideas) and society (public, politic). Switching between drought and floods, the cost of doughty, the multiple human pressures like the demography and the variability of fresh water resources in different regions are the themes which J. Sheffield(Princeton University, USA; see figure 4) talked about. The virtual water aspect and the ways about keeping natural balance for water quality in a mathematical model were presented by G. Bidoglio(EC-JRC IES Ispra, Italy; see figure 5). Realized Projects are the Joint Research Project SMART (integrated Water Resource Management in the Lower Jordan Valley) and the Guanting Project (sustainable water and Agricultural Land use in the Guanting watershed under limited water resources).

In summary the visit of the conference was productive in context about the main problem of water scarcity and the climate change which opened us new ways of thinking and point of views.

For the third year in series the German Ministry of Research and Education invited all German iGEM Teams to join the "Biotechnology 2020+ conference" in Berlin at June 27th http://www.biotechnologie2020plus.de "Biotechnology 2020 conference". “Which products and biotechnological processes are seminal? “ ,“How will the next generation accept the biotechnological processes?” and “Which projects are already initiated?” are a few discussed goals of the conference. Actors in the field of economy, politic and science participated in addition to the German iGEM teams at the conference. In the speech of Dr. Helge Braun (parliamentary State Secretary of the BMBF) who gave an overview of the milestones emphasized that a creative teamwork with an own developed language is necessary and introduced the new biotechnological ideas: biological implant, biomimetric desalination plant and microbial fuel cell. In the first discussion round about the topic “result of the previous activities and recommendations for action for the continuation of the initiative "Biotechnology 2020+ conference"" five ambassadors of five research institutions( Frauenhofer-corporation, Helmholtz-community, Max-Planck-corporation, Leibniz-community, TU Braunschweig) (see figure 6) presented their new realized ideas: printable biotechnology, cellfree bioproduction and synthesis of novel therapeutics. Exemplary Prof. Dr. H. Jäckle (Max-Planck-Community) introduced the research network for Synthetic Biology MaxSynbio. The second discussion round was concerned with “Open Source in the Biotechnology”. In general was recorded a split opinion in the audience. In addition to workshops with the main topic “On the way to a Biotechnology 2020 +” exhibition of the German IGEM projects 2013 (see figure 7) and presentations of the research projects of the five research institutions were also available.

In summary the participation enabled us to get inform about the practicable operated novel biotechnology ideas, cooperation and projects from market-leading research institutions in Germany on one side and relative to an international synthetic biology competition the different project of young German teams on the other side.

In Addition to present our project the goal of the visit of the Helmholtz Centrum of Leipzig UFZ http://www.ufz.de/index.php?en=2540 UFZ on the 26 th of june 2013 was to get another objective opinion concerning our project idea, project implementation and information about practical Phytoremediation. Dr. Kuschk and Dr. Müller are members of the chair of environmental biotechnology and attend to phytoremediation or rather bioremediation (see figure 6). Their research focus is in the area of zoonosis that means the interplay between plants and microorganisms. The plant acts with the root system as a carrier to quantify the microorganisms which dispose nitrate, sulfur or toluol. Because of lack of personal genetic engineering works in UFZ isn’t possible according to Dr. Kuschk.

Together with a leadership through the laboratories with Dr. Müller (see Figure 7) we had 2 conversations with the contact people in different points of our project. Dr. Müller gave us very helpful annotations relative to our effectors. He mentioned the fact about the sense of amplification of a resistance gene EreB in environment in contrast to the biotechnological benefit. Furthermore he alluded that we had to consider the enzyme specificity, to find out the benefit of extinction of Erythromycin relative to clean water and to inform us about the costs of blue light. Another point was the use of Cathecholdioxygenase. According to Dr. Müller is a depletion of Catechol through microorganisms a very effective process. So our duty was to show the efficiency of the depletion of Catechol through moss.

The main topic we discussed with Dr. Kuschk applied to the application of our project idea in practice outside the competition framework. Dr. Kuschk was in doubt about the practical conversion based on his long lasting experiences and compared to his own developed concepts and conversions. In his opinion the costs, the concentration of the influent and the high duration of dwell of water based on the low concentration of water play a negative role, effect and influence relative to the conversion. Furthermore he prefers biofilms to membrane concerning to the immobilization of substances. Also he criticized our choice of degraded substances. In his opinion fecal substance are the cause of the high rate of death and diseases in developed countries. We explained him that we choose harmful substances according to the WHO with respect to establish our filter and show with the help of few harmful substances the degradation and bioaccumulation of them. In general he saw our project as basic research and not as practical application. In summary the visit of the UFZ enriched us in the molecular genetics part and in the view of further project thoughts. The leadership through the laboratories of UFZ showed us exemplary demonstrative a conversion of phytoremediation.