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Team:Bielefeld-Germany/Project/Cytochromes
From 2013.igem.org
Cytochromes
Membranes can work as an isolator for the cell to prevent the transfer of charged molecules. So it is necessary to change the
membrane in a way that it can transfer the electrons without causing its collapse. The integration of cytochromes into the
membrane can help achieving a defined electron transport. A minimal set of cytochromes, consisting of the periplasmatic cytochrome MtrA, the outer membrane cytochrome MtrC and the outer membrane ß-barrel protein MtrB from Shewanella oneidensis MR-15 should be expressed in E. coli.
Mediators
Of great interest is the production of endogenous mediators. The overexpression of glyceroldehydrogenase in ''E. coli'' is a promising approach. Because many derivates of glyceroldehydrogenase are small, water-soluble redoxmolecules, they have the properties of a mediator. Futhermore, it will be tested, if there is a possibility of expressing the mediator phenazin. Phenazin is an endogenous mediator of ''Pseudomonas'' species.
