Calendar

General Inducer Characterization

June 2013
- Week 3 (24-30 June)
  Amplification of new biobricks for cloning:
  BBa_J23119: Strong promoter, BBa_P0440: rbs tetR term, BBa_I13521: Ptet rbs RFP term.
July 2013
- Week 4 (1-7 July)
  Assembly of a strong promoter with tetR. tetR protein in necessary to inhibits the Ptet promoter.
- Week 5 (8-14 July)
  Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of AHL.
  
  The construction is then sent for sequencing.
- Week 6 (15-21 July)
  The sequencing shows that the construction include a mutated promoter instead of J23119. So we assembled tetR with Ptet-RFP.
- Week 7 (22-28 July)
  We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.
August 2013
- Week 8 (29-4 August)
  The tries didn’t work, so we did it again one more week the assembly of Strong Promoter with tetR-Ptet-RFP.
- Week 9 (5-11 August)
  Because none of last clonages worked, we tried with several promoters in order to increase our chances of success.
- Week 10 (12-18 August)
  None of the passed assemblies worked, so we tried with a weak promoter.
- Week 11 (19-25 August)