Prototype

Initial Ideas

When we started thinking about how to turn our project into a product that could be readily used by the beef industry we wanted something that was portable and easy to use. Initial discussions with industry also pointed out that speed was important, as if it took 24 hours to get the result then the infected cow would have already been processed. To get inspiration we went for a walk around our local pharmacy to see what kind of sensors we could buy and explore. As we were walking through the aisles we saw digital and colour change thermometers, pressure based heart rate monitors, blood glucose meters, and pregnancy tests. While the idea of having a digital output was initially interesting to us, we soon realized that the home pregnancy test was one of the easiest and well known sensors with an output that can be understood by anyone. Another bonus was that the designs have already been optimized to use biological molecules as the recognition elements, just like we were planning to do.

With that in mind we sent two of our male advisors (Robert & Iain) to pick out their favourite pregnancy tests. The fruits of their labour can be seen below in Figure 1 (they even got two different kinds!).

Playing with Pregnancy Tests

After some very strange looks from our professors, we wanted to disassemble the tests we bought to see what made them tick. Before risking the integrity of the device though, our male prototypers wanted to watch it work, and thankfully the cup of water we dipped it in (no, we didn't get guys to pee on it) was not pregnant. With our curiosity sated we began pulling it apart. The components can be seen below in Figure 2

There were more components than we had expected, with an applicator, transfer wick, nitrocellulose, dragging wick, and a dessicator stone at the end. Through playing with the components we found that the dragging wick (after the nitrocellulose to pull the liquid sample as far as possible) was not completely necessary, but did seem to aid in covering the nitrocellulose. We believe the dessicator is for long term storage of the device, as it played no discernable role in our bench testing.

Testing Ferritin in Pregnancy Tests

To test if our system of using ferritin would give us a readable output from a prototype we blotted prussian blue ferritin onto nitrocellulose. We then enclosed the blotted nitrocellulose in one of the pregnancy tests along with the applicator and wicks that came with it before exposing it to the TMB substrate. The results of our first test can be seen below in Figure 3.

Unfortunately we didn't see any change between the before and after pictures, and after taking it apart to look inside we found that the solution had only flowed through approximately one third of the device. With that in mind we built our second prototype out of another pregnancy test, but this time we shortened the test by cutting a chunk of the applicator off. The downside of this modification was that the applicator was rendered useless, so filter paper, commonly used in western blots, was used instead. In this prototype, shown below in Figure 4, we were able to observe a change in prussian blue ferritin after the addition of the substrate TMB.