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Team:Macquarie Australia/Results
From 2013.igem.org
Results and Characterisation
This page aims to provide a summary of our most important successful results, which have provided large strides towards the develpment of goal; Chlorophyll synthesis within E. coli. An in-depth view of our results can be found in our Notebook
Primary Results
[Description]
Sequencing Results
[Description]
| BioBricks | Amino Acid Match | Match Percentage |
|---|---|---|
| POR | 317/319 | 99% |
| ChlG | 320/320 | 100% |
| ChlP | 421/427 | 99% |
| ChlM | 278/280 | 99% |
| YCF | 130/130 | 100% |
| CTH1 | 372/374 | 99% |
| ChlI1 | 353/353 | 100% |
| DVR1 | 299/300 | 99% |
| Plastocyanin | 97/97 | 100% |
| Gun4 | 216/216 | 100% |
Characterisation
Promoter was ligated onto the genes and plated up on LB + CAM plates, we observed a lot of colony growth. We inoculated LB + CAM broth with a colony from the coresponding plate. A variety of growth was observed, a few plates corresponding to a certain gense had a lot more colonies than others, this can be obesrved from the image with the description order of genes. More colonies coresponding to a gene displayed more colony growth than others which can be obesrved from the image description showing the order of genes on the agar.
