Prussian Blue Ferritin Synthesis

Reagents and Materials

• Horse Spleen Ferritin (47mg/mL)
• Hydrogen Chloride (pH 3.0)
• K₄Fe(CN)₆ (0.025M)
• Sodium acetate-acetic acid buffer (pH 3.6)

20µL of horsespleen ferritin (47mg/mL) was added to 20mL of HCl (pH 3.0) and 150µL of K₄Fe(CN)₆ (0.025M). The solution was kept under 4℃ for 12 hours while constantly being stirred.

After the 12 hour period, 5mL of the solution was dialyzed against 500mL of sodium acetate-acetic acid buffer (pH 3.6) for 24 hours, with the buffer being replaced after 8 hours.