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Team:Macquarie Australia/parts
From 2013.igem.org
The 2013 Macquarie iGEM team have continued the expansion of iGEM’s gene registry, adding a whole suite of genes integral to the chlorophyll biosynthesis pathway. We have designed biobrick versions of 13 genes, tweleve of which have been assembled. Nine of these biobricks have been sequenced to confirm fidelity to design and sent to iGEM headquarters, while we await the sequence confirmation of a further three. One final biobrick is still to be constructed.
Our intention of is to allow future teams the access to and documentation of genes, which were previously out of reach. The multitude of new genes we have added not only allows future team to continue with similar goals of harnessing photosynthetic properties, but to allow new and novel research tasks to be performed.
Parts Submitted - Under Construction
Our Team has successfully characterized the following parts and have submitted them to the registry:
Tetrapyrrole-binding protein - In Arabidopsis, GUN4 (Genomes uncoupled 4) is required for the functioning of the plastid mediated repression of nuclear transcription that is involved in controlling the levels of magnesium- protoporphyrin IX. GUN4 binds the product and substrate of Mg-chelatase, an enzyme that produces Mg-Proto, and activates Mg-chelatase. GUN4 is thought to participates in plastid-to-nucleus signaling by regulating magnesium-protoporphyrin IX synthesis or trafficking.
Electron transfer agent - In photosynthesis, plastocyanin functions as an electron transfer agent between cytochrome f of the cytochrome b6f complex from photosystem II and P700+ from photosystem I. Cytochrome b6f complex and P700+ are both membrane-bound proteins with exposed residues on the lumen-side of the thylakoid membrane of chloroplasts. Cytochrome f acts as an electron donor while P700+ accepts electrons from reduced plastocyanin. [Condense This, Stolen directly from Wikiwikiwikipedia]
In addition to the above parts which were submitted to the registry, we have also developed other parts that have not been submitted. This includes all the genes necessary to develop chlorophyll biosynthesis within E.coli. Details of all our developed parts can be found below.
