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Team:TU-Delft/Protocol 3
From 2013.igem.org
Protocols
Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.
- Transforming Parts from Distribution kit
- Growing the Single Colonies from the Agar Plates
- Making glycerol stocks
- Miniprep Protocol
- Restriction digestion
- Ligation
- Gel Extraction Procedure
- PCR Purification
3. Making glycerol stocks:
Requirements:
1. Glycerol 2. Pipettes 3. 1.5 mL Tubes 4. Culture SampleProdecure:
1. Label the tubes and add 180μL of glycerol.2. Pipette out 1000 μL of the culture.
3. Add the culture to the tubes and store in -80°C
The glycerol stock can be used whenever required, by just adding 0.5 mL of stock into 5 mL of freshly prepared media.
