Team:TMU-Tokyo/Notebook experiment/1st

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Experiment > Parts Synthesis > 1st week


September 2nd
PCR following fragment.
FragmentPtetR1PtetR2FLPSmaraC-P
PrimerCmNR-stu
FLP-IRR/IRL		CmCR-stu
FLP-IRR/IRL		FLP-L
FLP-R		FLP-SaApL
CmC		acaC-2
BAD-C3
TempleteIRR/IRL-GFP
(BBa_K880002)		IRR/IRL-GFP
(BBa_K880002)		pFTGTm(C2-Ap)SmpBAD/Myc-HisA
FragmenthbiFTclacIFRT-CmFRT-Ap
PrimerpBAD-HbiF
RTcN-hbiF		TcN
TcC3		141-70
FRT-lacIC		R6Kori-Xho-3R
R(FRT)2		lacI-R(FRT)2
mhpA-SaApR
TempletehbiF
(BBa_K880000)		Escherichia coli
MG1655 recA⁻
(cell suspention)		Escherichia coli
MG1655 genome		pSG76CpSG76A2

Result

1: PterR1, 2: PtetR2, 3: FLP, 4: Sm, 5: araC-P,
6: hbiF, 7: Tc, 8: LacI, 9: FRT-Cm, 10: FRT-Ap


September 3rd
Vector Remodeling with PCR. Insertion Recognition sites (Blunt).
FragmentKm-Blunt
PrimerKm-Blunt-N
Km-Blunt-C
TempletepACYC177

Result



DNA Purification following fragment from gel.
PtetR1
FLP
Sm
araC-P
hbiF
Tc
lacI
FRT-Cm
FRT-Ap
Km-Blunt


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