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Team:TMU-Tokyo/Notebook experiment/4th
From 2013.igem.org
Experiment > 4th week
September 22th
Mini prep following plasmid.
| fimE-pBAD(in pSB1A3), pSB1C3-Km, pSB1A3-Km |
1: pSB1C3-Km, 2-4: pSB1A3-Km, 5-6: fimE-pBAD
Check PCR.
| Fragment | (control) | Fragment 3 - ApCm |
| Primer | 141-49 SaApL | 141-49 SaApL |
| Templete | Escherichia coli MG1655 red (Cell suspention) | (Cell suspention) |
1-9: Fragment 3 - ApCm
Check PCR.
| Fragment | (control) | Fragment 3 - ApCm | Fragment 3 - ApCm |
| Primer | 2 sets of the others | 141-1 141-20 | 141-1 141-74 |
| Templete | Escherichia coli MG1655 red (Cell suspention) | (Cell suspention) |
1-7: 141-1 and 141-20, 8: control(141-1 and 141-20),
9-15: 141-1 and 141-74, 16: control(141-1 and 141-74),
Digestion following plasmid.
| pSB1C3-Km-Blunt |
P1 Preparation following fragment.
| Fragment 1, 2 |
September 23th
September 24th
September 25th
September 26th
September 27th
September 28th
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