Week 15: 23rd September - 29th September

Microfluidic experiment

30/09/13
Chip's channel were full of bacteria, even after 2 hours of washing (with free iron M9) because we let the bacteria growth over the week-end (in LB medium). Hence, we prepared another chip to launch another experiment on the next morning.
As our bacteria have a plasmid AceB-sfGFP and there is no iron in our M9, we still check if bacteria expressed sfGFP protein.
The first results appear promising, but we need to confirm them with a proper experiment.

Figure 1: Escherichia coli (TOP 10 strain) expressing sfGFP.
At the top of the picture, we can see that the channel is full of bacteria.