Team:TU-Munich/Results/BioBricks

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  • Igkappa-GFP

<groupparts>iGEM2013 TU-Munich</groupparts> <groupparts>iGEM2013 Dundee</groupparts>

Figure 1:Successful preparing of the biobrick sendbox
Figure 2:One of our biobrick sendboxes

BioBricks we improved

Some BioBricks which were already present in the parts registry were converted from RFC[10] to RFC[25] by PCR. The purpose of this improvement was that we introduced with our project the possiblility to dictate the cellular localisation of proteins (cytosolic, secreted and receptor bound). For this purpose the open reading frame of the protein has to be fused to a N-terminal signal peptide (for secretion) or has to be inserted into the extracellular domain of a synthetic receptor. We converted the following BioBricks to RFC[25]:

  • Laccase <partinfo>BBa_K863000</partinfo> was improved to the BioBrick <partinfo>BBa_K1159002</partinfo>
  • Protein phosphatase 1

For the fluoresceine binding anticalin

  • FluA

BioBricks we characterized

  • LMU-GFP

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.

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