Journal

Milestones

The iGEM-Team Bielefeld 2013 started working and decided to deal with the project 'Microbial Fuel Cell'.

Initiating labwork on the sub-project endogenous mediator Glycerol dehydrogenase.
Starting lab work with the first successful PCR's. The main work however is still in the planning of our project. Designing experiments and a lot of research.
Finding sponsors goes ahead. Many companies like our project and want to support us.

Starting labwork on the sub-project Porins.
Successful PCR on OprF gene from Pseudomonas fluorescens. OprF with Pre- and Suffix overlaps could be amplified from genome.
Planning of our Human Practice projects started and the first participations are fixed.

Poster presentation at the congress ‘[http://www.biotechnologie2020plus.de Next generation of biotechnological processes 2020+]’ in Berlin.
Sponsoring acquisition is nearly finished.
Problem with cloning delaying the progress in the laboratory.
We have published our [http://ekvv.uni-bielefeld.de/blog/uniaktuell/entry/mit_bakterien_batterie_strom_erzeugen first press release] with a great response. The press release was picked up worldwide.

Our great expert Dr. Falk Harnisch has answered numerous questions about our project and helped us very well.
GldA BioBrick (<bbpart>BBa_K1172201</bbpart>) was examined.
- GldA Biobrick Devices with different promotors and RBS could be added: (<bbpart>BBa_K1172203</bbpart>, <bbpart>BBa_K1172204</bbpart>, <bbpart>BBa_K1172205</bbpart>)
OprF BioBrick (<bbpart>BBa_K1172501</bbpart>) is available.
- OprF Biobrick was functionalized with different promotors and RBS: (<bbpart>BBa_K1172502</bbpart>, <bbpart>BBa_K1172503</bbpart>, <bbpart>BBa_K1172504</bbpart>, <bbpart>BBa_K1172505</bbpart>, <bbpart>BBa_K1172507</bbpart>)
Successful protein expression and overproduction of glycerol dehydrogenase. SDS-PAGE shows GldA at expected size.