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From 2013.igem.org

Labbook |	Protocols |	Materials |

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

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Contents 1 Strains 2 Media 3 Antibiotics 4 Enzymes 4.1 Phosphatases 4.2 DNA polymerases 4.3 DNA ligases 4.4 Restriction enzymes 5 Electrophoresis 5.1 Agarose gel electrophoresis 5.2 PAGE 6 Buffers 7 Induction 8 DNA sequences

Strains

• [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
• [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
• [http://ecoliwiki.net/colipedia/index.php/BL21(DE3) BL21DE3]

Media

• DYT
• LB
• SOB
• SOC

Antibiotics

• Ampicillin
• Chloramphenicol
• Kanamycin

Enzymes

Phosphatases

• Antarctic Phosphatase

DNA polymerases

• Phusion
• Taq Polymerase
• Q5 Polymerase
• 5x InFusion Pfu MasterMix

DNA ligases

• T4 DNA ligase

Restriction enzymes

• NheI
• EcoRI
• PstI
• SpeI
• XbaI

Electrophoresis

Agarose gel electrophoresis

• TriTrack loading dye
• DNA ladders
• Nancy
• TAE

PAGE

• Coomassie stain
• Destain
• SDS-Page

Buffers

• PBS
• TE
• Tris

Induction

• Arabinose
• IPTG

DNA sequences

• Plasmids
• Sequences
• Primer