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From 2013.igem.org

All the protocols on this page have been extensively tested to verify which parts matter and which don't. Your results may vary but please try this before changing things.

To begin with create chemically competent cell using Chemically Competent Cells creation protocol.

Next these cells should be transformed using Chemical Transformations Protocol

Test Competence to make sure they work well use this transformation protocol

Resusspend the RE parts from the parts kit

Transform parts

Miniprep

Whole cell lysate to digest with

Digest your part using this protocol

Ligate using this protocol

Transform results