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Team:TU-Delft/Notebook/2013/07/25/
From 2013.igem.org
Notebook
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25th of July
Lab work
1. The Peptides : Magainin and Signiferin showed good results. The plates plated again.
2. DNA extraction of pT7, SUMO and Ulp1 was carried out.
3. Colony PCR on pTET:cI:TT was done. The ligation product was transformed into XL1 blue cells.
4. Stapyllococcus delphini and Bacillus subtilis has grown well on the plates.
5. Quick change of SUMO and Ulp 1 was done at annealing temperatures 60 degree and 55 degree and transformed into XL1 Blue cells.
