Requirements for a Bronze Medal

Register the team, have a great summer, and plan to have fun at the Regional Jamboree.

Successfully complete and submit this iGEM 2013 Judging form.

Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.

Plan to present a Poster and Talk at the iGEM Jamboree.

Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry. Part Number(s):

• [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)
• [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)
• [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)

Description of function

Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

Quantitative data showing the Part or Device function

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908). Acknowledgment of sources and references

Additional Requirements for a Silver Medal

Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.

We measure our superfolder proteins and they fluorescent that we expected.

Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry

Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)

Part Number(s):

• [http://parts.igem.org/Part:BBa_K1093000 K1093000]
• [http://parts.igem.org/Part:BBa_K1093001 K1093001]
• [http://parts.igem.org/Part:BBa_K1093002 K1093002]

Other parts that we characterized here

Description of function

Quantitative data showing the Part or Device function

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

Acknowledgment of sources and references

Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.

Link to page on your team's wiki: Safety

Additional Requirements for a Gold Medal (one OR more)

We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.

Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).

The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards

Part Number(s):

• [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)
• [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)
• [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)

Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.

• [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.

Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

Description of function

Quantitative data showing the Part or Device function

Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

Acknowledgment of sources and references

Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.

Link to page on your team's wiki: Cooperation with other iGEM teams

Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.

iGEM Prizes

Best Human Practice Advance

Please explain briefly why you should receive any of these special prizes:

• We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN.
• We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology See more
• We cooperated with mass media. See more at: Cooperation with mass media