Team:BYU Provo/Notebook/Cholera - Enzymes/September-October

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Cholera - Enzyme Notebook September-October
Cholera Enzyme March-April May-June July-August September-October Protocols	September 1 - September 14 We successfully prepared the DspB and AmyA plasmids to submit to the iGem registry and those to be used in our protein purification. We then were able to purify DspB and AmyA to use in our biofilm assay. Daily log
	September 15 - September 30 We focused on running our biofilm assay and getting data on the degradative enzymatic activity of the DspB and AmyA proteins we have purified. Daily log
	October 1 - October 14 We went to the North American iGem Jamboree and did well enough to make it to the World Championships in November. We got back to the lab extremely excited and motivated. We worked on finalizing our DspB plasmid to get it ready to over express and purify in E. coli. Daily log
	October 15 - October 31 We finished purifying DspB and AmyA, ran Biofilm Assays to determine the effect of these enzymes on the biofilm of V. cholerae, and planned future assays and experiments to continue this area of research. Daily log