Team:Macquarie Australia/Notebook

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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.


Notebook
To browse through our notebook simply click on the image with the date. It will expand with what we did that week. To continue on simply click on the next one. If the notebook isn't functioning as planned (it will not load the next tab of content) then please go to our linearised view which is located here.



Week 1- Tuesday July 31st

With the break between semesters over, the Macquarie iGEM team returned to classes; for us this was the first day of iGEM 2012. We had our first meeting and discussed our project. Dr. Louise Brown and Associate Professor Rob Willows were introduced to the team and we began to determine who would take on certain roles within the team.

We eagerly began our project, deciding to use the novel approach of Gibson Assembly to produce our optimised genes. We had decided to develop a gene-switch controlled by light. To do this a couple of genes would be required:

  1. A bacteriophytochrome
  2. Heme oxygenase

Gibson Assembly

The bacteriophytochromes from Deinococcus radiodurans and Agrobacterium tumefaciens were chosen. Over the next week Matt Stclair started to develop the Gibson Blocks (gBlocks) by:

  1. Acquiring the DNA sequence
  2. Translating into the protein sequence
  3. Optimising codon usage for E. coli using the DNA sequence
  4. Translating into protein sequence and ensuring no changes in amino acids relative to the original sequence

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