Team:CU-Boulder/Notebook/Protocols
From 2013.igem.org
- Embedding PDF Files
- In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
- Embedding Google Docs
- Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
- Preparing Chemically Competent Cells
- This protocol details how to prepare chemically competent cells.
- Chemical Transformations
- In this protocol we cover how to use our chemically competent cells to perform transformations.
- Plasmid Miniprep
- Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
- Restriction (Double) Digests
- Our method of performing restriction double digests is detailed in this protocol.