Team:CU-Boulder/Notebook/Protocols

Embedding PDF Files

In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.

Embedding Google Docs

Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.

Chemically Competent Cells

This protocol details how to prepare chemically competent cells.

Chemical Transformations

In this protocol we cover how to use our chemically competent cells to perform transformations.

Plasmid Minipreps

Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.

Restriction (Double) Digests

Our method of performing restriction double digests is detailed in this protocol.

Recycling DNA Mini-columns

This protocol covers how to recycle mini-columns used in plasmid miniprep systems.

Protein Miniprep

Digest Lysate

Ligation