Transformation

Goal

In this step, the heat shock (between -80°C up to 42°C) induces bacteria to be in state of competence. DNA from the environment (in our case: plasmid) can then integrate the cell.

Preparation

For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. If the ligation protocol is a Golden Gate , add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.

Controles

To check if the transformation have work, make a positive and a negative controle.