Notebook : April 20

General meeeting

Week end Brainstorming : 20&21 April 2013

People there : Instructors and advisors : Sylvie, Philippe, Nguyen, Solenne Students : Pierre-Louis, Gabriel, Zhou, Nima, Andy, Damir, Kevin, Jean-Rémy, Xavier, Caroline, Anaïs, Eric, Abdou.

Saturday 20th April :

1. Presentation of the simulation’s software of Damir

This is a htlm page that Damir did to follow any biological systems. This simple software can be use by everybody if they know the different constants of their system. (adresse ip) - Very good idea, we can develop it at the same time that the scientific project - Look if others software were done by igem teams - Find constants in literacy - Find people to help Damir

2. Proposition of projects

a) Caroline’s Project : Seine’s depollution

Problematic: PCBs are toxic, ecotoxic, carcinogenic and presents in the Seine in sediments.

The idea: create a biological system that can detect and degrade PCBs Others iGEM team worked on it : Clemson 2012, Lethbridge 2008 Bielfield 2012

Questions: - What chassis ?

- What genes and enzymes ? burkoderia, pseudomonas, http://www.ncbi.nlm.nih.gov/pubmed/23529656

- Can it possible to do ? Not to hard ? there a lot a genes and enzymes to combine

- Kind of action of the PCBs ?

- Safety : how to limit the spreading of the bacteria? Gemote ?

Pros and cons :

+ - Seine = our environment to save Natural bacteria did it, why do it with E.Coli ? Burkoderia Xenovorans : one way, 2 gens : easier Can it work ? Complete subject : chemistrie, biology, ethics … PCBs are in sediments, how to degrade it ? Concentration of PCBs in water too small

Contacting ideas : Mr. Dubow to visit a water treatment plant http://www.futura-sciences.com/fr/news/t/developpement-durable-1/d/pcb-la-frnce-contaminee_31534 http://www.futura-sciences.com/fr/news/t/developpement-durable-1/d/pollution-fluviale-au-pcb-une-etude-sonne-lalerte_15709

b) Nima’s Project

Problematic: Cambridge 2007 BOL : Bacteria Online https://2007.igem.org/wiki/index.php/Project

Idea : Make a analogy between internet and synbiology : intern and extern communication. Creation of an amplification system of flux with a binary system

Questions: - Degradation of the signal

- Order of the signals

- Storage of the signal

- How to restart the signal ?

Pros and Cons : + - Utility ?

c) N Guyen’s proposition : detection of Cs

Questions :

- Receptors known ?

- Reaction that can be developed ?

Pros and cons : + - Tc and LA : radioactive forms Can we make the difference between Cs and radioactive Cs ? Cs present in our environment

Sunday 21th April :

Voting

Seine pollution biobits Biotime keeper Cs detection Votes number 13 5 0 0 arguments We can make it Interesting reflexion on the project for HP Interdisciplinary Social problem Attractive for sponsors Interesting We can take over biobricks Original

Depollution Project adopted !