"
Team:Bielefeld-Germany/Labjournal/July
From 2013.igem.org
July
Milestones
1.Week
MFC
Mediators
Cytochromes
The mtrCAB fragment has two illegal PstI restriction sites at x xbp and yy bp, so we had to design new primers to remove them. We replaced one base in each restriction site, without affecting the coding triplett, by respective primer overlaps . We ended up with three different fragments, which will be ligated back together via Gibson Assembly.
mtrCAB_Frag1_rev:
mtrCAB_Frag2_fwd:
mtrCAB_Frag2_rev:
mtrCAB_Frag3_fwd:
Amplification of the first fragment for Gibson Assembly
Program: PhusionPCR
mtrC_fwd:
mtr_Frag1_rev:
Amplification of the second fragment for Gibson Assembly
Program: PhusionPCR
mtr_Frag2_fwd:
mtr_Frag2_rev:
Amplification of the third fragment for Gibson Assembly
Program: PhusionPCR
mtr_Frag3_fwd:
mtr_B_rev:
