Bradford Assay

Reagents and Materials

• 96-well plate
• Bradford Reagent
• BSA standards (e.g., 0.2mg/mL, 0.5mg/mL, 1.0mg/mL and 1.5mg/mL)
• Protein samples
• Spectrophotometer

Protocol

1. Add 20µL of standards (in triplicates) and samples into the wells
2. Add 200µL of Bradford Reagent to each well and incubate at room temperature for 10 min
3. Read the absorbance at 595nm
4. Plot a standard curve to obtain a linear equation
5. Use the linear equation to calculate the protein concentration of each sample