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Team:TU-Delft/Notebook/2013/07/17/
From 2013.igem.org
Notebook
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17th of July
Lab work
1. AIP receiver:GFP and pTET:RBS:cI was ligated and grown in Agar plates, then cultured, DNA extracted.
2. Running gel electrophoresis on the restriction digestion of AIP receiver:GFP with XbaI +PstI and pTET:RBS:cI with EcoRI +SpeI.
2. Checking for Quick change on SUMO and Ulp 1, which was not successful at PCR annealing temperature at 50 degree and 55 degree.
3. Transformation of pT7(BBa_I719005) in DH5Alpha cells was carried out.
