Team:BYU Provo/Notebook/Phage Purification/Springexp/Period1/Dailylog

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May 13 - May 26 Daily Log'''</font>
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May 1 - May 12 Daily Log'''</font>
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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="4"> '''5/29/13''' </font>
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- Finished the PEG purification.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.26_PEG_Purification|5.26 PEG Purification]]
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<font size="4"> '''5/31/13''' </font>
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<font size="4"> '''5/1/13''' </font>
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- Worked on setting up our notebook online.
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: Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing
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<font size="4"> '''6/3/13''' </font>
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<font size="4"> '''5/3/13''' </font>
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- Ran the CsCl purification step.
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: We created an Excel sheet to calculate the amounts of each reagent for the phage purification experiment we will be running on Monday. This will allow for quick and  easy calculations of reagents needed each time we run the experiment.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
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- Next class we will attempt extracting the phage from the gradient.
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<font size="4"> '''6/5/13''' </font>
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<font size="4"> '''5/5/13''' </font>
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: Today we created fresh bacteria to work with
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- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle. 
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.5 Bacteria Cultures, 25 mL |5.5 Bacteria Cultures, 25 mL]]
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: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
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:In the results, W3110 failed to grow, so the experiment will have to be repeated.
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: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
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: We also centrifuged bacterial lysate infected with phage in order to store the phage for future use.  
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: Placed these in the refrigerator.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.6 Phage From Lysate |5.6 Phage From Lysate]]
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: We are waiting on materials to begin running the phage purification experiments.
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- Prepared stocks of bacteria for future use.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
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<font size="4"> '''3/20/13''' </font>
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<font size="4"> '''5/8/13''' </font>
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: New liquid cultures of both W3110 and BL21 were created following the steps in [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.8 Bacteria Cultures, 25 mL |5.8 Bacteria Cultures, 25 mL]]
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: After 1 hour of growth, BL 21 had T7 phage added to allow 48 hours infection time. 
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.8 Infect With Phage |5.8 Infect With Phage]]
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<font size="4"> '''3/22/13''' </font>
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<font size="4"> '''5/10/13''' </font>
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:We made a W3110 liquid culture
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:  [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.10 Bacteria Cultures, 25 mL |5.10 Bacteria Cultures, 25 mL]]
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:We emailed Dr. McCleary on ultracentrifuge training.
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<font size="4"> '''3/25/13''' </font>
 
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<font size="4"> '''5/24/13''' </font>
 
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- Ran a CsCl gradient to further purify phage from
 
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<font size="4"> '''5/26/13''' </font>
 
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- Began the PEG purification.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.26_PEG_Purification|5.26 PEG Purification]]
 
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Latest revision as of 00:02, 28 September 2013


Phage Purification May - June Notebook: May 1 - May 12 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



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5/1/13

Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing


5/3/13

We created an Excel sheet to calculate the amounts of each reagent for the phage purification experiment we will be running on Monday. This will allow for quick and easy calculations of reagents needed each time we run the experiment.


5/5/13

Today we created fresh bacteria to work with
5.5 Bacteria Cultures, 25 mL
In the results, W3110 failed to grow, so the experiment will have to be repeated.
We also centrifuged bacterial lysate infected with phage in order to store the phage for future use.
5.6 Phage From Lysate
We are waiting on materials to begin running the phage purification experiments.


5/8/13

New liquid cultures of both W3110 and BL21 were created following the steps in 5.8 Bacteria Cultures, 25 mL
After 1 hour of growth, BL 21 had T7 phage added to allow 48 hours infection time.
5.8 Infect With Phage


5/10/13

We made a W3110 liquid culture
5.10 Bacteria Cultures, 25 mL
We emailed Dr. McCleary on ultracentrifuge training.







<< Previous Next >>

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